Generator

Part:BBa_K2638758:Design

Designed by: Antonin Lenzen   Group: iGEM18_Bielefeld-CeBiTec   (2018-10-10)


Seamless siRNA Expression Vektor for Omp-Utr protected siRNAs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The vector was created without scars to minimize changes in the transkribed siRNA through placeholder bases.


Source

Gene Synthesis, 2018 Distribution Plates.

References